Optimization of trace elements in purified glycerol for microbial lipid and citric acid production by Yarrowia lipolytica SKY7

作     者：Lalit R.Kumar Sravan K.Yellapu R.D.Tyagi Patrick Drogui 

作者机构：INRS-ETE490Rue de la CouronneQuebecQC G1K 9A9Canada 

出 版 物：《Systems Microbiology and Biomanufacturing》 (系统微生物学与生物制造（英文）)

年 卷 期：2021年第1卷第1期

页      面：76-89页

学科分类：0202[经济学-应用经济学] 02[经济学] 020205[经济学-产业经济学] 

基　　金：Natural Sciences and Engineering Research Council of Canada, NSERC, (A4984) Canada Research Chairs 

主　　题：Yarrowia lipolytica SKY7 Purified glycerol Trace elements Microbial lipid Citric acid 

摘      要：Purified glycerol obtained after acid treatment of crude glycerol solution was used as the carbon source for lipid and citric acid production using *** *** purified glycerol was high in phosphorus,it was important to inves-tigate the impact of fortification of trace elements in the medium on cell growth,lipid and citric acid(CA)*** all the trace elements(including phosphates and sulfates)required for growth and lipid production were added to the purified glycerol medium,high biomass(51.67 g/L)and lipid concentration(19.47 g/L)were observed at 96 h of fed-batch fermentation with low CA concentration of 5.42 g/*** purified glycerol medium without additional trace elements gave low biomass(27.67 g/L),lipid concentration(9.35 g/L)at 80 h of fed-batch fermentation,but gave high CA concentration(24.51 g/L).When purified glycerol was provided with only sulfates or all elements except KH_(2)PO_(4),low biomass(32.59 g/L and 38.52 g/L)and citric acid concentration(1 g/L and 2.42 g/L)were obtained at 96 h.