Stem cell transplantation and/or adenoviral glial cell line-derived neurotrophic factor promote functional recovery in hemiparkinsonian rats

作     者：May-Jywan Tsai Shih-Chieh Hung Ching-Feng Weng Su-Fen Fan Dann-Ying Liou Wen-Cheng Huang Kang-Du Liu Henrich Cheng 

作者机构：Department of NeurosurgeryNeurological InstituteNeurological InstituteTaipei 11217Taiwan Department of Medical ResearchNational Yang Ming UniversityInstitute of Clinical MedicineTaipei 112Taiwan Integrative Stem Cell CenterDepartment of OrthopaedicsChina Medical University HospitalTaichung 404Taiwan Institute of Biomedical SciencesAcademia SinicaTaipei 11529Taiwan China Medical UniversityGraduate Institute of Clinical Medical SciencesTaichung 404Taiwan Department of Medical Research and EducationTaipei Veterans General HospitalStem Cell LaboratoryTaipei 112Taiwan Department of Life ScienceInstitute of BiotechnologyHaulien 97401Taiwan Department of NeurosurgeryNeurological InstituteTaipei 112Taiwan School of MedicineNational Yang-Ming UniversityTaipei 112Taiwan Department of neurosurgeryNeurological InstituteTaipei 112Taiwan Department of NeurosurgeryTaipei Veterans General HospitalCenter for Neural RegenerationNeurological InstituteTaipei 112Taiwan 

出 版 物：《World Journal of Stem Cells》 (世界干细胞杂志（英文版）（电子版）)

年 卷 期：2021年第13卷第1期

页      面：78-90页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100204[医学-神经病学] 10[医学] 

基　　金：Supported by Taipei Veterans General Hospital in Taiwan,No.V106C-012,No.V107C-087,and No.V109C-018 and Ministry of Science and Technology in Taiwan,No.MOST106-2314-B-075-023,No.MOST107-2314-B-010-023,and No.MOST107-2314-B-075-021 

主　　题：Stem cells Transplantation Parkinson’s disease Glial cell line-derived neurotrophic factor Adenovirus Neuroregeneration 

摘      要：BACKGROUND Parkinson’s disease(PD)is a neurological disorder characterized by the progressive loss of midbrain dopamine(DA)*** marrow mesenchymal stem cells(BMSCs)can differentiate into multiple cell types including neurons and *** of BMSCs is regarded as a potential approach for promoting neural *** cell line-derived neurotrophic factor(GDNF)can induce BMSC differentiation into neuron-like *** work evaluated the efficacy of nigral grafts of human BMSCs(hMSCs)and/or adenoviral(Ad)GDNF gene transfer in 6-hydroxydopamine(6-OHDA)-lesioned hemiparkinsonian *** To evaluate the efficacy of nigral grafts of hMSCs and/or Ad-GDNF gene transfer in 6-OHDA-lesioned hemiparkinsonian *** We used immortalized hMSCs,which retain their potential for neuronal ***,preinduced hMSCs,or Ad-GDNF effectively enhanced neuronal connections in cultured *** vivo,preinduced hMSCs and/or Ad-GDNF were injected into the substantia nigra(SN)after induction of a unilateral 6-OHDA lesion in the nigrostriatal *** Hemiparkinsonian rats that received preinduced hMSC graft and/or Ad-GDNF showed significant recovery of apomorphine-induced rotational behavior and the number of nigral DA ***,DA levels in the striatum were not restored by these therapeutic *** hMSCs might reconstitute a niche to support tissue repair rather than contribute to the generation of new neurons in the injured *** The results suggest that preinduced hMSC grafts exert a regenerative effect and may have the potential to improve clinical outcome.