In-vitro assessment for the control of Fusarium species using a lactic acid bacterium isolated from yellow pitahaya(Selenicereus megalanthus(K. Schum. Ex Vaupel Moran))

作     者：Leidy J.VALENCIA-HERNANDEZ Karina LÓOPEZ-LÓPEZ Eyder D.GÓMEZ-LÓPEZ Liliana SERNACOCK Cristobal N.AGUILAR Leidy J.VALENCIA-HERNáNDEZ;Karina LóPEZ-LóPEZ;Eyder D.GóMEZ-LóPEZ;Liliana SERNA-COCK;Cristobal N.AGUILAR

作者机构：Universidad Nacional de ColombiaPalmira CampusValle 763533Colombia Food Research DepartmentSchool of ChemistryAutonomous University of CoahuilaSaltillo CampusCoahuila 25280Mexico 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2021年第20卷第1期

页      面：159-167页

核心收录：

学科分类：0710[理学-生物学] 0832[工学-食品科学与工程（可授工学、农学学位）] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0905[农学-畜牧学] 09[农学] 0906[农学-兽医学] 0904[农学-植物保护] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 0713[理学-生态学] 

基　　金：the Administrative Department of Science, Technology, and Innovation, Colciencias, Asoppitaya the Inter-American Development Bank IDB for funding。 

主　　题：lactic acid bacteria Fusarium fungistatic activity biomass yellow pitahaya 

摘      要：The fungistatic activity of a lactic acid bacterium, which had been isolated from yellow pitahaya cultures, against fungi associated with basal rot(Fusarium oxysporum and Fusarium fujikuroi) was measured in the present study. Its activity was assessed in three fractions: fermented(S1), metabolic products(S2), and biomass(S3), using two fermentation substrates: Man Rogosa Sharpe agar(MRS) and potato dextrose agar(PDA). The bacterium was molecularly identified as Lactobacillus plantarum. S3 reduced F. fujikuroi growth by 100% over 48 h of fermentation, which occurred during the stationary phase of bacterial growth. The three fractions fungistatic activity against F. fujikuroi depended on the substrate employed. The fermentation kinetic parameters for L. plantarum indicated that its specific growth rate was 0.46 h^–1, with 93.63% substrate consumption, 0.045 kg kg^–1 cell yield, and 0.54 kg kg^–1 product yield. The kinetic parameters calculated will allow for bacteria production scaling. These in-vitro trials reveal L. plantarum s possible application as a biocontrol agent for diseases associated with Fusarium. However, further ex-vivo and in-vivo researches are required to demonstrate its behavior in crops.