Isolation and characterization of a high-efficiency marine diesel oil-degrading bacterium

作     者：Jin Gao Jie Ming Meng Xu Xinge Fu Liang-Feng Duan Cong-Chao Xu Yu Gao Jian-Liang Xue Xin-Feng Xiao Jin Gao;Jie Ming;Meng Xu;Xinge Fu;Liang-Feng Duan;Cong-Chao Xu;Yu Gao;Jian-Liang Xue;Xin-Feng Xiao

作者机构：College of Safety and Environmental EngineeringShandong University of Science and TechnologyQingdao266590China College of Chemical EngineeringChina University of PetroleumBeijing102249China 

出 版 物：《Petroleum Science》 (石油科学（英文版）)

年 卷 期：2021年第18卷第2期

页      面：641-653页

核心收录：

学科分类：083002[工学-环境工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 08[工学] 09[农学] 0903[农学-农业资源与环境] 0713[理学-生态学] 

基　　金：This work was supported by the Scientific Research Foundation of Shandong University of Science and Technology under Grant for Recruited Talents[2014RCJJ015] Project of Shandong Province Higher Educational Young Innovative Talent Introduction and Cultivation Team[Hydrogen energy chemistry innovation team]and the Science and Technology Projects of Qingdao(Grant No.19-6-2-25-cg) 

主　　题：Bioremediation Bacillus megaterium MJ4 Environmental factors Marine oil spill Response surface methodology 

摘      要：In the recent 50 years,marine oil spills had resulted in severe environmental pollution problems *** this study,12 petroleum-degrading strains named MJ1 to MJ12,which can use diesel oil as the sole carbon source for growth,were isolated from the seawater in Jiaozhou Bay,*** MJ4 has the highest diesel-degrading rate which is up to 26.54%in 5 days with the diesel oil concentration of 10 g/*** to the BLAST research,16SrRNA sequence of MJ4 showed 99%similarity to Bacillus megaterium ***-factor experiments and response surface methodology were carried out to optimize the environmental factors and their reciprocal action for affecting the diesel oil degradation process of Bacillus *** of single-factor experiments revealed that the highest degradation rate was obtained with temperature of 28°C,pH of 8.8,diesel oil concentration of 25 g/L,P/N ratio of 0.56,nitrogen and phosphorus dosage quantity of 0.35 g/L and 0.18 g/L,respectively.A nonlinear regression equation of diesel oil degradation rate and pH,temperature,P/N ratio was *** model predicted the maximum degradation rate of 72.21%with temperature of 28°C,pH of 8.88,P/N ratio of 0.31,respectively.