Identification and functional prediction of long intergenic noncoding RNAs in fetal porcine longissimus dorsi muscle

作     者：LI Cen-cen YU Shu-long REN Hai-feng WU Wei WANG Ya-ling HAN Qiu XU Hai-xia XU Yong-jie ZHANG Peng-peng LI Cen-cen;YU Shu-long;REN Hai-feng;WU Wei;WANG Ya-ling;HAN Qiu;XU Hai-xia;XU Yong-jie;ZHANG Peng-peng

作者机构：College of Life SciencesXinyang Normal UniversityXinyang 464000P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2021年第20卷第1期

页      面：201-211页

核心收录：

学科分类：0710[理学-生物学] 0832[工学-食品科学与工程（可授工学、农学学位）] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0905[农学-畜牧学] 09[农学] 0906[农学-兽医学] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 0713[理学-生态学] 

基　　金：financially supported by the National Natural Science Foundation of China (31601167, 31972537 and U1204326) the Natural Science Foundation of Henan Province, China (182300410027) the Central Plains Technological Innovation Leading Talents Project of Henan Province, China (194200510022) the Nanhu Scholars Program of Xinyang Normal University, China。 

主　　题：lincRNAs pig skeletal muscle embryo potential target genes 

摘      要：Pigs are globally farmed animals which provide protein for human consumption in the form of skeletal muscle. To better understand the function of long intergenic noncoding RNAs(linc RNAs) in porcine skeletal muscle growth and development, we collected RNA-seq data from porcine longissimus dorsi muscle(LDM) during embryonic development. We identified a total of 739 linc RNA transcripts, which were distributed on all chromosomes except the chromosome Y, and analyzed their molecular characteristics. Compared to protein-coding genes, linc RNAs showed shorter transcripts, longer exons, fewer exons and higher tissue specificity. In addition, the abundance of linc RNAs in five embryonic development stages were analyzed and 45 differentially expressed linc RNAs were screened, three of which were highly expressed in LDM during porcine embryonic development. Finally, we predicted the potential target genes and functions of the linc RNAs, and identified 1 537 cis-target genes and 8 571 trans-target genes. Furthermore, we identified two key candidate linc RNAs involved in muscle development, XLOC_024652 and XLOC_001832, for post-trial validation. Our results provide a genome-wide resource of linc RNAs which are potentially involved in porcine embryonic skeletal muscle development and lay a foundation for the further study of their functions.