Jianpi Huazhuo Tiaozhi granules reduce oxidative stress injury in macrophages by inhibiting the nicotinamide adenine dinucleotide phosphate oxidase/reactive oxygen species-nuclear transcription factor kappa B pathway

作     者：Liu Yanwei Liu Zhongyong Liu Yanwei;Liu Zhongyong

作者机构：School of MedicineJiangxi University of Traditional Chinese MedicineNanchang 330004China Department of CardiologyAffiliated Hospital of Jiangxi University of Traditional Chinese MedicineNanchang 330006China 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2020年第40卷第6期

页      面：922-927页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1005[医学-中医学] 1002[医学-临床医学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by National Natural Science Foundation of China Jianpi Huazhuo Tiaozhi Granules Based on the Theory of Turbidity Adjust and Control NOX/ROS-NF-k B Signaling Pathway and the Mechanism for the Treatment of Atherosclerosis(No.81660781) the Innovation Special Fund Project of Graduate Students Jiangxi Province Study on the Mechanism of Treating Atherosclerosis by Jianpi Huazhuo Tiaozhi Granules(No.YC2017-B085) 

主　　题：Atherosclerosis Oxidative stress Macrophages NADPH oxidases Reactive oxygen species NF-kappa B Jianpi Huazhuo Tiaozhi granules 

摘      要：OBJECTIVE:To assess the effect of Jianpi Huazhuo Tiaozhi granules(JHTG)on oxidative stress damage to macrophages and explore the relationship between the levels of this damage and the nicotinamide adenine dinucleotide phosphate oxidase(NOX)/reactive oxygen species(ROS)-nuclear transcription factor kappa B(NF-κB)signaling ***:Macrophages cultured in vitro were divided into seven groups:control,model control,inhibitor,positive control,2.5%JHTG,5%JHTG,and10%*** oxidative stress injury model was established by stimulating macrophages with oxidized low-density *** survival and apoptosis were detected by 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide and flow cytometry assays,*** and superoxide dismutase levels were detected by enzyme-linked immunosorbent assays,while ROS levels were detected using a fluorescence *** and m RNAs associated with the NOX/ROS-NF-κB pathway,including NOX4,p22 phox,inhibitor of NF-κB kinase-α(IKK-α),inhibitor of NF-κB kinase-β(IKK-β),and NF-κB were detected by Western blot and PCR,***:After JHTG treatment,there were fewer damaged and apoptotic macrophages,while superoxide dismutase levels were *** JHTG-treated groups also showed reduced ROS *** molecular changes following JHTG treatment included decreased expression of NOX4 and p22 phox at the protein level and decreased IKK-α,IKK-β,and NF-κB expression at the m RNA *** of these effects were correlated with the JHTG ***:These results demonstrated that the molecular mechanism underlying the antioxidant activity of JHTG in macrophages is through inhibiting the NOX/ROS-NF-κB pathway.