Combination of hollow fiber-based liquid-phase microextraction with sweeping techniques in micellar electrokinetic chromatography for the determination of Strychnos alkaloids in human urine

作     者：Xiao Huan Zang Cai Rui Li Qiu Hua Wu Chun Wang Dan Dan Han Zhi Wang 

作者机构：Key Laboratory of Bioinorganic Chemistry College of Science Agricultural University of Hebei Baoding 071001 China College of Vocational and Technological Sanitation Hebei University Baoding 071002 China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2007年第18卷第3期

页      面：316-318页

核心收录：

学科分类：1010[医学-医学技术（可授医学、理学学位）] 0703[理学-化学] 10[医学] 

基　　金：This work was supported both by the Natural Science Foundation of Hebei Province(B2006000413) the Scientific Research Foundation for the Returned 0verseas Chinese Scholars,State Education Ministry 

主　　题：Hollow fiber-based liquid-phase microextraction Sweeping MEKC Strychnos alkaloids Urine 

摘      要：A new method for the enrichment of Strychnos alkaloids in biological samples via liquid-phase microextxaodon （LPME） based on porous polypropylene hollow fibers in combination with on-line sweeping in micellar electrokinodc chromatography was developed. The calibration curve was linear over the range of 20-200 ng mL^-1 for both strychnine and brucine in human urine sample. The detection limits （S/N = 3：1） for strychnine and brucine were 1 ng mL^-1 and 2 ng mL^-1, respectively. The LPME-sweeping method has been successfully applied to the analysis of strychnine and brucine in real urine samples.