Changes of matrix metalloproteinases in the stroma after corneal cross-linking in rabbits
作者机构：Department of Ophthalmologythe Sixth Medical Center of Chinese PLA General HospitalBeijing 100048China
出 版 物：《International Journal of Ophthalmology(English edition)》 (国际眼科杂志（英文版）)
年 卷 期：2021年第14卷第1期
页 面：26-31页
核心收录：
学科分类：1002[医学-临床医学] 100212[医学-眼科学] 10[医学]
基 金：Supported by Beijing Municipal Science and Technology Commission(No.Z151100004015217)
主 题：corneal cross-linking keratoconus rabbit riboflavin matrix metalloproteinase
摘 要：AIM:To observe changes in the content of matrix metalloproteinases(MMPs)in the corneal stroma after corneal cross-linking(CXL)in rabbits,and further explore the corneal pathophysiological process after ***:Forty-two rabbits(42 eyes)were randomly divided into seven *** group served as the control group,while the other six groups were treated with *** concentrations of MMPs in corneal stroma were evaluated through parallel reaction monitoring at baseline and 3,7,15,30,90,and 180 d after ***:The levels of MMP-2 in the corneal stroma of rabbits were 0.76±0.07,2.78±1.39,4.12±0.69,2.00±0.29,2.00±0.30,1.22±0.18,and 1.35±0.18(10^(-9)mol/g)at baseline and 3,7,15,30,90,and 180 d after treatment,*** contents of tissue inhibitor of metalloproteinase-1(TIMP-1)were 1.83±0.26,7.94±0.58,6.95±2.64,3.81±0.48,3.07±0.92,1.72±0.19,and 1.69±0.74(10^(-9)mol/g),*** ratios of MMP-2/TIMP-1 were 0.42±0.33,0.36±0.20,0.62±0.10,0.54±0.15,0.68±0.13,0.71±0.10,and 0.68±0.09,*** CXL,the expression of MMP-2 and TIMP-1 in the rabbit corneal stroma was initially increased and subsequently *** levels of MMP-2 remained higher than those recorded at baseline 180 d after treatment,but it was not statistically *** levels of TIMP-1 returned to baseline levels at 90 d after *** ratio of MMP-2/TIMP-1 started to rise from 7 d after *** was significantly higher than that calculated at baseline 30-180 d after *** results for MMP-1,-3,-7,-9,-13,and TIMP-2 were ***:CXL can lead to changes in the content of MMP-2 and TIMP-1 in the rabbit corneal *** ratio of MMP-2/TIMP-1 remains higher versus baseline,indicating that MMP-2 is involved in the corneal pathophysiological process after CXL.
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