Genome-wide distribution and functions of the AAE complex in epigenetic regulation in Arabidopsis

作     者：Yi-Zhe Zhang Juncheng Lin Zhizhong Ren Chun-Xiang Chen Daisuke Miki Si-Si Xie Jian Zhang Ya-Nan Chang Jing Jiang Jun Yan Qingshun Q.Li Jian-Kang Zhu Cheng-Guo Duan Yi-Zhe Zhang;Juncheng Lin;Zhizhong Ren;Chun-Xiang Chen;Daisuke Miki;Si-Si Xie;Jian Zhang;Ya-Nan Chang;Jing Jiang;Jun Yan;Qingshun Q.Li;Jian-Kang Zhu;Cheng-Guo Duan

作者机构：Shanghai Center for Plant Stress Biology and CAS Center of Excellence for Molecular Plant SciencesChinese Academy of SciencesShanghai 201602China University of Chinese Academy of SciencesBeijing 100049China Key Laboratory of the Ministry of Education for Coastal and Wetland EcosystemsCollege of the Environment and EcologyXiamen UniversityXiamen 361102China State Key Laboratory of Crop Stress Adaptation and ImprovementSchool of Life SciencesHenan UniversityKaifeng 475004China School of Life SciencesEast China Normal UniversityShanghai 200241China Graduate College of Biomedical SciencesWestern University of Health SciencesPomona 91766USA Department of Horticulture and Landscape ArchitecturePurdue UniversityWest Lafayette 47907USA 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2021年第63卷第4期

页      面：707-722页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071007[理学-遗传学] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 

基　　金：supported by the Chinese Academy of Sciences,including the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB27040203 to C.-G.D.and XDB27040000 to J.K.Z) in part by a grant from the National Key Research and Development Project of China(2016YFE0108800 to Q.Q.L.)。 

主　　题：alternative polyadenylation alternative splicing epigenetic regulation heterochromatin transcription 

摘      要：Heterochromatin is widespread in eukaryotic genomes and has diverse impacts depending on its genomic context.Previous studies have shown that a protein complex,the ASI1-AIPP1-EDM2(AAE)complex,participates in polyadenylation regulation of several intronic heterochromatin-containing genes.However,the genome-wide functions of AAE are still unknown.Here,we show that the ASI1 and EDM2 mostly target the common genomic regions on a genome-wide level and preferentially interacts with genetic heterochromatin.Polyadenylation(poly(A)sequencing reveals that AAE complex has a substantial influence on poly(A)site usage of heterochromatin-containing genes,includingnotonlyintronicheterochromatincontaining genes but also the genes showing overlap with heterochromatin.Intriguingly,AAE is also involved in the alternative splicing regulation of a number of heterochromatin-overlapping genes,such as the disease resistance gene RPP4.We provided evidence that genic heterochromatin is indispensable for the recruitment of AAE in polyadenylation and splicing regulation.In addition to conferring RNA processing regulation at genic heterochromatin-containing genes,AAE also targets some transposable elements(TEs)outside of genes(including TEs sandwiched by genes and island TEs)for epigenetic silencing.Our results reveal new functions of AAE in RNA processing and epigenetic silencing,and thus representimportantadvancesinepigenetic regulation.