Mitochondria are Main Targets of Time/Dose-Dependent Oxidative Damage-Based Hepatotoxicity Caused by Rhizoma Dioscoreae Bulbiferae in Mice

作     者：Hong-Ping Hou Guang-Ping Zhang Han Li Teng-Fei Chen Yun-Hang Gao Ling Song Zhong-Xiu Zhang Zu-Guang Ye Hong-Ping Hou;Guang-Ping Zhang;Han Li;Teng-Fei Chen;Yun-Hang Gao;Ling Song;Zhong-Xiu Zhang;Zu-Guang Ye

作者机构：Atomization Inhalation Research CenterInstitute of Chinese Materia MedicaChina Academy of Chinese Medical SciencesBeijingChina 

出 版 物：《World Journal of Traditional Chinese Medicine》 (世界中医药杂志（英文）)

年 卷 期：2020年第6卷第4期

页      面：461-468页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by The National Key Research and Development Plan 2019YFC1712403 Major New Drug Discovery Science and Technology Major Projects 2017ZX09201002-006 the Research Project of Capital Health and Development 2018-4-4231 

主　　题：Correlation hepatotoxicity mitochondria oxidative damage Rhizoma Dioscoreae bulbiferae 

摘      要：Background:Rhizoma Dioscoreae bulbiferae could cause liver damage,which limited its application in the *** and Objectives:To explore the mechanism of hepatotoxicity of Rhizoma Dioscoreae bulbiferae in *** and Methods:In the present study,the water extraction of Rhizoma Dioscoreae bulbiferae(W.E.R)was administrated via intragastrical with Low(19.6 g/kg),Middle(28.0 g/kg),and High(40.0 g/kg)dose in *** each time point 14 days,21 days,and 28 days,the body weight,liver coefficient,indexes of liver function alkaline phosphatase(ALP),alanine aminotransferase(ALT)and aspartate transaminase(AST),various biochemical biomarkers of liver tissue and mitochondria were detected and ***:We found that W.E.R could decrease the body weight,increase the liver coefficient and the expression levels of indexes of liver function in ***,we investigated that W.E.R could increase the expression levels of reactive oxygen species(ROS)and malondialdehyde(MDA),decrease the expression levels of adenosine triphosphate(ATP)and improve the enzyme activities of total superoxide dismutase(SOD).Third,we found that W.E.R could increase the enzyme activities of manganese-superoxide dismutase,decrease the enzyme activities of sodium-potassium adenosine triphosphatase(Na+-K+-ATPase)and calcium-magnesium adenosine triphosphatase(Ca 2+-Mg 2+-ATPase).Finally,we analyzed that there were significant negative correlations between body weight,expression level of ATP,activity of Na+-K+-ATPase,activity of Ca 2+-Mg 2+-ATPase and time,*** were significant positive correlations between liver coefficient,ALP,ALT,AST,expression levels of ROS,MDA and time,***:All the results above indicated that W.E.R could cause the hepatotoxicity based on the oxidative damage in mice,which and mitochondria might be the main targets.