Involvement of TRPC1 and Cyclin D1 in Human Pulmonary Artery Smooth Muscle Cells Proliferation Induced by Cigarette Smoke Extract
作者机构：Department of Respiratory and Critical Care MedicineRenmin Hospital of Wuhan UniversityWuhan 430060China
出 版 物：《Current Medical Science》 (当代医学科学（英文）)
年 卷 期：2020年第40卷第6期
页 面：1085-1091页
核心收录：
学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 0703[理学-化学] 100214[医学-肿瘤学] 10[医学]
主 题：cigarette smoke extract human pulmonary artery smooth muscle cells transient receptor potential channel 1 cyclin D1
摘 要：Cigarette smoking contributes to the development of pulmonary artery hypertension(PAH).As the basic pathological change of PAH,pulmonary vascular remodeling is considered to be related to the abnormal proliferation of pulmonary artery smooth muscle cells(PASMCs).However,the molecular mechanism underlying this process remains not exactly *** aim of this research was to study the molecular mechanism of PASMCs proliferation induced by *** PASMCs(HPASMCs)were divided into 6 groups:0%(control group),cigarette smoking extract(CSE)-treated groups at concentrations of 0.5%,1%,2%,5%,10%CSE *** proliferation was observed after 24 *** were divided into two groups:0(control group),0.5%CSE *** mRNA and protein expression levels of transient receptor potential channel 1(TRPC1)and cyclin D1 in HPASMCs after CSE treatment were respectively detected by RT-PCR and Western *** intracellular calcium ion concentration was measured by the calcium probe in each *** the negative control group and TRPC1-siRNA transfection group,the proliferation of HPASMCs and the expression of cyclin D1 mRNA and protein were *** were compared with one-way ANOVA(for multiple-group comparison)and independent t-test(for two-group comparison)followed by the least significant difference(LSD)test with the computer software SPSS *** was found that 0.5%and 1%CSE could promote the proliferation of HPASMCs(P0.05),and the former was more effective than the latter(P0.05),while 3%and above CSE had inhibitory effect on HPASMCs(P0.05).The mRNA and protein expression levels of TRPC1 and cyclin D1 in 0.5%and 1%CSE groups were significantly higher than those in the control group(P0.05),while those in 3%CSE group were significantly decreased(P0.05).Moreover,the proliferation of HPASMCs and the expression of cyclin D1 mRNA and protein in TRPC1-siRNA transfection group were significantly reduced as compared with those in the negative control group(P
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