Peripheral nerve regeneration with cotransplantation of umbilical cord mesenchymal stem cells and Schwann cells in rat sciatic nerve defect

作     者：Jin-Yong Lee Hun-Jong Jung Mohammad S. Alrashdan Bohan Li Mi-Ae sung Sang BaeYoo Soung-Min Kim Myung-Jin Kim Jeong Won Jahng Jong-Ho Lee 

作者机构：Department of Oral and Maxillofacial Surgery School of Dentistry Seoul National University Seoul Korea Department of Occupation and Environment Konkuk Postgraduate Medical School Choong-Ju Korea Department of Craniofacial Structure and Functional Biology School of Dentistry Seoul National University Seoul Korea Dental Research Institute School of Dentistry Seoul National University Seoul Korea 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2011年第6卷第7期

页      面：485-493页

核心收录：

学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学] 

基　　金：the Korea Healthcare Technol-ogy R&D Project  Ministry for Health  Welfare & Family Affairs  Republic of Korea  No. A101578 

主　　题：Schwann cells human umbilical cord mesenchymal stem cells peripheral nerve re-generation axotomy 

摘      要：Previous research has demonstrated that cotransplantation of umbilical cord mesenchymal stem cells （UCMSCs） and Schwann cells （SCs） can repair spinal nerve injury, but few studies have investigated their use in peripheral nerve regeneration. In the present study, we cotransplanted UCMSCs and SCs to repair 5-mm left sciatic nerve defects in rats, and compared the effects of UCMSCs ＋ SCs transplantation with UCMSCs or SCs transplantation alone. After UCMSCs ＋ SCs transplantation, nerve conduction velocity of the left sciatic nerve and gait were both improved. Retrograde tracing analysis demonstrated that the mean count of fluorogold-labeled neurons, as well as the mean axon count and axon density, were significantly greater in the left sciatic nerve after UCMSCs ＋ SCs transplantation, compared with UCMSCs or SCs transplantation alone. Improvements in conduction velocity and increased sheath thickness in the left sciatic nerve were similar after UCMSCs transplantation and UCMSCs ＋ SCs transplantation. These findings suggest that UCMSCs transplantation can promote the repair of sciatic nerve defects to some extent, but that combined UCMSCs ＋ SCs transplantation has a significantly greater regenerative effect.