Inheritance Analysis and Identification of SSR Markers Linked to Late Blight Resistant Gene in Tomato

作     者：ZHU Hai-shan WU Tao ZHANG Zhen-xian 

作者机构：Department of Vegetable College of Agronomy and Bio-Technology China Agricultural University Beijing 100094 P.R.China Department of Horticulture College of Landscape and Horticulture Yunnan Agricultural University Kunming 650201 P.R.China 

出 版 物：《Agricultural Sciences in China》 (中国农业科学（英文版）)

年 卷 期：2006年第5卷第7期

页      面：517-521页

学科分类：09[农学] 0901[农学-作物学] 

基　　金：Key Project of 10th Five-Year Plan of Yunnan Provincial Government  (2001NG21) 

主　　题：disease resistance late blight Phytophthora infestans SSR tomato 

摘      要：Late blight caused by Phytophthora infestans is the most serious disease of tomato production in China. Studies on the genetics of resistance and identification of molecular markers are very useful for breeding late blight resistant varieties. The objective of this paper was to study the inheritance of late blight resistance and identify simple sequence repeat （SSR） markers associated with resistance allele in tomato （Lycopersicon esculentum Mill）. The results came from an F2 progeny of 241 plants derived from a cross between 5~ inbred line that is susceptible to late blight and a resistant accession CLN2037E. The late blight responses of F2 plants were tested by artificially inoculation of detached-leaflets in plate and natural infection assayed under greenhouse conditions. Both methods showed that the resistance is dominant and inherited as monogenic trait. Genetic mapping and linkage analysis showed that the late blight resistance gene Ph-ROL was located on chromosome 9 with a genetic distance of 5.7 cM to the SSR marker TOM236.