Efficiency of DNA barcodes for species delimitation:A case in Pterygiella Oliv.(Orobanchaceae)

作     者：Alexandra H.WORTLEY 

作者机构：Royal Botanic Garden Edinburgh 

出 版 物：《Journal of Systematics and Evolution》 (植物分类学报(英文版))

年 卷 期：2011年第49卷第3期

页      面：189-202页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：supported by the National Natural Science Foundation of China(Grant No.30970201) the Research Fund for the Large-scale Scientific Facilities of CAS(Grant No.2009-LSF-GBOWS-01) 

主　　题：compensatory base changes(CBCs) DNA barcode ITS-2 non-monophyletic species Pterygiella Oliv. secondary structure. 

摘      要：DNA barcoding is becoming an increasingly popular means to identify *** obscure discrimination in the genus Pterygiella calls into question the re-assessment of the criterion for species *** collected 20 individuals,representing all five described species of this genus in its distributional *** aim was to use three proposed barcode DNA regions(rbcL,matK,and ITS) to diagnose Pterygiella species,and examine which barcode is more suitable for discerning the congeneric and related *** results showed that the core barcodes matK and rbcL were comparatively less ***,the ITS region,especially ITS-1 and ITS-2,successfully identified all species in the ***,the secondary structure of ITS-2 RNA,especially compensatory base changes,appears complementary to classical primary sequence analysis for DNA barcoding.