皮肤淋巴细胞增生性疾病患者发生人疱疹病毒8感染

作     者：Trento E Castilletti C Ferraro C P. Cordiali-Fei 李晓莉 

作者机构：Laboratory of Clinical Pathology San Gallicano Dermatology Institute Via Elio Chianesi 53 00144 Roma Italy.Dr 

出 版 物：《世界核心医学期刊文摘（皮肤病学分册）》 (Digest of the World Core Medical JOurnals:Dermatology)

年 卷 期：2006年第2卷第1期

页      面：27-28页

学科分类：1002[医学-临床医学] 100206[医学-皮肤病与性病学] 10[医学] 

主　　题：疱疹病毒 蕈样肉芽肿 副银屑病 斑块型 免疫性皮肤病 淋巴瘤 血清学方法 经典型 血清病毒载量 免疫 

摘      要：Objective: To investigate the prevalence of human herpesvirus 8 (HHV-8; Kaposi sarcoma-associated herpes-virus) infection in patients with lymphoproliferative skin diseases such as large plaque parapsoriasis (LPP) and mycosis fungoides compared with inflammatory cutaneous conditions or healthy control subjects. Design: A survey study was undertaken in 123 subjects with various clini cal conditions. Setting: All patients had been seen in the Dermatology Departmen t of the San Gallicano Dermatology Institute, Rome, Italy, in the last 2 years. Patients: Forty-five patientswith inflammatory or autoimmune cutaneous diseases , 50 healthy control subjects, 10 patients with LPP, 12 patients with mycosis fu ngoides, and 6 patients with classic Kaposi sarcoma were included in the study. Main Outcome Measures: The prevalence of HHV-8 infectionwas investigated with s erologic studies using the gold standard assay based on body cavity-based B-cell lymphoma-1 cells latently infected with HHV-8. The presence of HHV-8 conserved sequence, corresponding to open reading frame 26, was also assessed in the peripheral blood and lesion tissue samples from patients with lymphoproliferative cutaneous diseases with nested polymerase chain reaction. The presence and distribution of cell types infected with HHV-8 in the lesion tissues was determined with immunohistochemical staining with the monoclonal antibody directed against the latent nuclear antigen-1 of HHV-8 encoded by open reading frame 73. Results: In healthy control subjects and patients with inflammatory skin diseases, 13.9%were found to have antibody against HHV-8, consistent with the seroprevalence population in Italy. A highly significant association of HHV-8 infection and LPPwas found (100%) compared with mycosis fungoides (25%). The peripheral blood mononuclear cells in 8 of 10 patients with LPP were found to harbor viral sequences at nested polymerase chain reaction, whereas none of them had a detectable serum viral load. All