Intestinal organoids as tools for enriching and studying specific and rare cell types:advances and future directions

作     者：Kim E.Boonekamp Talya L.Dayton Hans Clevers Kim E.Boonekamp;Talya L.Dayton;Hans Clevers

作者机构：Oncode InstituteHubrecht InstituteRoyal Netherlands Academy of Arts and Sciences(KNAW)and University Medical Centre(UMC)Utrecht358A CT UtrechtThe Netherlands Princess Maxima Centre for Paediatric Oncology358A CS UtrechtThe Netherlands 

出 版 物：《Journal of Molecular Cell Biology》 (分子细胞生物学报（英文版）)

年 卷 期：2020年第12卷第8期

页      面：562-568页

核心收录：

学科分类：1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 10[医学] 

基　　金：This work was supported by the European Research Council under ERC Advanced Grant Agreement no.67013(H.C.and K.B.)。 

主　　题：directions organo rare 

摘      要：Adult tissue-derived organoids allow for the expansion and maintenance of primary epithelial cells in a near-native state.These 3D and self-organizing organotypic cultures derived from adult tissues have been increasingly used in fundamental and translational research.A key feature of this organoid system is that it recapitulates the stem cell lineage and thus,the differentiated cell-type heterogeneity of the in vivo tissue of origin.