Three-dimensional biofabrication of an aragoniteenriched self-hardening bone graft substitute and assessment of its osteogenicity in vitro and in vivo
作者机构：Union Hospital affiliated to Tongji Medical College of Huazhong University of Science and TechnologyWuhanHubei ProvinceChina Centre for NanohealthSwansea University Medical SchoolSwanseaUK Centre for NanohealthFaculty of Science and EngineeringSwansea UniversitySwanseaUK State Key Laboratory of Materials Processing and Die&Mould TechnologyHuazhong University of Science and TechnologyWuhanHubei ProvinceChina
出 版 物：《Biomaterials Translational》 (生物材料转化电子杂志（英文）)
年 卷 期：2020年第1卷第1期
页 面：69-81页
学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学]
基 金：This study was supported by the Wuhan International Collaboration Project of China(No.2017030209020252) Wuhan Science and Technology Project of China(No.2018010401011281)
主 题：biofabrication cytotoxicity hydroxyapatite/aragonite osteogenesis
摘 要：A self-hardening three-dimensional(3D)-porous composite bone graft consisting of 65 wt%hydroxyapatite(HA)and 35 wt%aragonite was fabricated using a 3D-Bioplotter®.New tetracalcium phosphate and dicalcium phosphate anhydrous/aragonite/gelatine paste formulae were developed to overcome the phase separation of the liquid and solid *** mechanical properties,porosity,height and width stability of the end products were optimised through a systematic analysis of the fabrication processing parameters including printing pressure,printing speed and distance between *** resulting 3D-printed bone graft was confirmed to be a mixture of HA and aragonite by X-ray diffraction,Fourier transform infrared spectroscopy and energy dispersive X-ray *** compression strength of HA/aragonite was between 0.56 and 2.49 *** was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay in *** osteogenicity of HA/aragonite was evaluated in vitro by alkaline phosphatase assay using human umbilical cord matrix mesenchymal stem cells,and in vivo by juxtapositional implantation between the tibia and the anterior tibialis muscle in *** results showed that the scaffold was not toxic and supported osteogenic differentiation in ***/aragonite stimulated new bone formation that bridged host bone and intramuscular implants in *** conclude that HA/aragonite is a biodegradable and conductive bone formation biomaterial that stimulates bone *** this material is formed near 37°C,it will have great potential for incorporating bioactive molecules to suit personalised application;however,further study of its biodegradation and osteogenic capacity is *** study was approved by the Animal Ethical Committee at Tongji Medical School,Huazhong University of Science and Technology(IACUC No.738)on October 1,2017.
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