Extrahepatic bile duct reconstruction in pigs with heterogenous animal-derived artificial bile ducts:A preliminary experience

作     者：Hao Shang Jian-Ping Zeng Si-Yuan Wang Ying Xiao Jiang-Hui Yang Shao-Qing Yu Xiang-Chen Liu Nan Jiang Xia-Li Shi Shuo Jin 

作者机构：Department of Hepatopancreatobiliary SurgeryBeijing Tsinghua Changgung HospitalSchool of Clinical MedicineTsinghua UniversityBeijing 102200China Department of PathologyBeijing Tsinghua Changgung HospitalSchool of Clinical MedicineTsinghua UniversityBeijing 102200China Institute for Precision Medicine Tsinghua UniversityBeijing Tsinghua Changgung HospitalSchool of Clinical MedicineTsinghua UniversityBeijing 102218China Department of Anesthesiology and OperationThe Second Affiliated Hospital of Xi’an Jiaotong UniversityXi’an 710004Shaanxi ProvinceChina 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2020年第26卷第46期

页      面：7312-7324页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：the Beijing Natural Science Foundation No.7194338 

主　　题：Bile duct injury Animal-derived artificial bile duct Heterogenous ureteral graft Nonimmunogenic Bile duct reconstruction Common bile duct 

摘      要：BACKGROUND Extrahepatic biliary duct injury(BDI)remains a complicated issue for *** several approaches have been explored to address this problem,the high incidence of complications affects postoperative *** a nonimmunogenic scaffold,an animal-derived artificial bile duct(ada-BD)could replace the defect,providing good physiological conditions for the regeneration of autologous bile duct structures without changing the original anatomical and physiologic *** To evaluate the long-term feasibility of a novel heterogenous ada-BD for treating extrahepatic BDI in *** Eight pigs were randomly divided into two groups in the *** animal injury model was developed with an approximately 2 cm segmental defect of various parts of the common bile duct(CBD)for all pigs.A 2 cm long novel heterogenous animal-derived bile duct was used to repair this segmental defect(group A,ada-BD-to-duodenum anastomosis to repair the distal CBD defect;group B,ada-BD-to-CBD anastomosis to repair the intermedial CBD defect).The endpoint for observation was 6 mo(group A)and 12 mo(group B)after the *** function was regularly *** were euthanized at the above *** analysis was carried out to assess the efficacy of the *** The median operative time was 2.45 h(2-3 h),with a median anastomosis time of 60.5 min(55-73 min).All experimental animals survived until the endpoints for *** liver function was almost *** analysis indicated a marked biliary epithelial layer covering the neo-bile duct and regeneration of the submucosal connective tissue and smooth muscle without significant signs of immune *** comparison,the submucosal connective tissue was more regular and thicker in group B than in group A,and there was superior integrity of the regeneration of the biliary epithelial *** the advantages of the regeneration of the bile duct smooth muscle observed in group A,th