Mageb4,a Testis-Specific Gene,is Dispensable for Mouse Spermatogenesis
Mageb4, a Testis-Specific Gene, is Dispensable for Mouse Spermatogenesis作者机构：Medical SchoolInstitute of Reproductive MedicineNantong UniversityNantong 226001China
出 版 物：《Reproductive and Developmental Medicine》 (生殖与发育医学（英文版）)
年 卷 期：2020年第4卷第3期
页 面：129-136页
核心收录：
学科分类：0710[理学-生物学] 1002[医学-临床医学] 10[医学]
基 金：the National Key Research and Development Program of China(No.2018YFC1003500 to F.S) the Postgraduate Research and Practice Innovation Program of Jiangsu Province(KYCX19_2027)。
主 题：Clustered Regularly Interspaced Palindromic Repeats/CRISPR-Associated Protein 9 Mageb4 Male Infertility Spermatogenesis
摘 要：Objective:It has recently been shown that the melanoma antigen gene(MAGE)family is expressed in various tumor cell lines but silent in normal tissues,except germ cell lines.Mageb4,a member of the MAGE family,is highly expressed in the testis and homologous in humans and mice.Whole-exome sequencing studies have identifiedMageb4 as a possible X-linked cause of inherited male infertility.However,the function of Mageb4 protein remains largely unknown.Methods:Using clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein(Cas)9 technology,we generated aMageb4 knockout mouse model(Mageb4^-/Y)to explore the role of this gene in spermatogenesis.Results:First,immunostaining of testicular cells showed thatMageb4 is localized in the cytoplasm of spermatogonia.Second,Mageb4^-/Y male mice displayed significant increases in apoptosis.However,Mageb4^-/Y male mice showed normal fertility,including normal sperm concentration,sperm motility,and testicular and epididymal histology.Conclusions:These findings suggest that,despite testis-exclusive expression,Mageb4 is dispensable for mouse spermatogenesis.Future research should focus on the role of this gene in apoptosis,aiming to provide clinical guidance regarding male infertility.
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