Vanadium-dependent activation of glucose transport in adipocytes by catecholamines is not mediated via adrenoceptor stimulation or monoamine oxidase activity

作     者：Jessica Fontaine Geneviève Tavernier Nathalie Morin Christian Carpéné 

作者机构：Institut des Maladies Métaboliques et CardiovasculairesInstitut National de la Santéet de la Recherche MédicaleINSERM UMR1048UniversitéPaul Sabatier Toulouse IIIToulouse 31432France INSERM UMR 1139 Facultéde PharmacieUniversitéde ParisParis 75006France 

出 版 物：《World Journal of Diabetes》 (世界糖尿病杂志（英文版）（电子版）)

年 卷 期：2020年第11卷第12期

页      面：622-643页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 1002[医学-临床医学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：We thank the staff of animal unit CREFRE  currently headed by Xavier Collet  andespecially its Rangueil satellite for housing wild type and transgenic rodents  andAnne Bouloumié for helpful discussions 

主　　题：Adipocyte Amine oxidases Insulin Diabetes Vanadium Obesity 

摘      要：BACKGROUND Benzylamine and methylamine activate glucose uptake in *** tyramine,this effect has even been extended to *** To investigate the effects of catecholamines and other amines on glucose *** A screening compared 25 biogenic amines on 2-deoxyglucose(2-DG)uptake activation in rat *** approaches and transgenic mouse models were then used to decipher the mode of action of several *** In rat adipocytes,insulin stimulation of 2-DG uptake was reproduced with catecholamines.100μmol/L or 1 mmol/L adrenaline,noradrenaline,dopamine and deoxyepinephrine,maximally activated hexose transport only when sodium orthovanadate was added at 100μmol/*** activation was similar to that already reported for benzylamine,methylamine and tyramine,well-recognized substrates of semicarbazide-sensitive amine oxidase(SSAO)and monoamine oxidase(MAO).Several,but not all,tested agonists ofβ-adrenoreceptors(β-ARs)also activated glucose transport whileα-AR agonists were *** of blockade byα-andβ-AR antagonists indicated that catecholamine-induced 2-DG uptake was not mediated by AR *** from mice lackingβ1-,β2-andβ3-ARs(triple KO)also responded to millimolar doses of adrenaline or noradrenaline by activating hexose transport in the presence of 100μmol/L *** MAO blocker pargyline,and SSAO inhibitors did not block the effects of adrenaline or noradrenaline plus vanadate,which were blunted by *** Catecholamines exert unexpected insulin-like actions in adipocytes when combined with *** limiting insulin resistance by activating glucose consumption at least in fat stores,we propose that catecholamine derivatives combined with vanadium can generate novel complexes that may have low toxicity and promising anti-diabetic properties.