Topical application of adipose tissuederived mesenchymal stem cells(ADMSCs)reduced cerebral edema in experimental traumatic brain injury(TBI)-a preliminary study

作     者：Hui Ma Lian Xu Cui Ping Kuen Lam Cindy S.W.Tong Kin K.Y.Lo George K.C.Wong Wai Sang Poon Ma Hui;Cui Lian Xu;Lam Ping Kuen;Tong Cindy S. W.;Lo Kin K. Y.;Wong George K. C.;Poon Wai Sang

作者机构：Division of NeurosurgeryChow Tai Fook-Cheng Yu Tung Surgical Stem Cell Research CenterDepartment of SurgeryThe Chinese University of Hong KongShatinHong KongSARChina Division of NeurosurgeryDepartment of SurgeryFo Shan First People’s HospitalFoshanGuangdongChina 

出 版 物：《Chinese Neurosurgical Journal》 (中华神经外科杂志（英文）)

年 卷 期：2021年第7卷第2期

页      面：131-136页

核心收录：

学科分类：1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 1009[医学-特种医学] 10[医学] 

基　　金：This research did not receive any specific grant from funding agencies in the public  commercial  or not-for-profit sectors 

主　　题：Topical MSCs Cerebral edema TBI 

摘      要：Background:Our previous studies showed that topical application of mesenchymal stem cells(MSCs)improved functional recovery in rat traumatic brain injury(TBI)model,and hypoxic precondition further enhanced the therapeutic effects of *** was no previous study on the attenuation of cerebral edema by *** investigated whether topical application of normoxic and hypoxic MSCs could reduce cerebral edema in an experimental TBI ***:Two million normoxic(N=24)and hypoxic(N=24)MSCs were applied topically to exposed the cerebral cortex in a controlled cortical impact(CCI)*** MSCs were fixed in position with fibrin *** treatment was given to control animals(TBI only:n=24).After surgery,four animals in each group were sacrificed daily(day 1 to day 6)for edema *** animals without TBI were used as reference(n=4).The expressions of GFAP,AQP4,and MMP9 were also investigated by immunofluorescence staining and RT-PCR at day ***:The edema peaked within 3 days after *** with the control,hypoxic MSCs reduced brain water content significantly(p0.05).Both hypoxic and normoxic MSCs downregulated the expression of MMP9 and normalized AQP4 distribution to astrocyte end ***:Our preliminary study showed that topical application of hypoxic MSCs suppressed both vasogenic and cytotoxic edema formation.