Utility of direct 3D co-culture model for chondrogenic differentiation of mesenchymal stem cells on hyaluronan scaffold(Hyaff-11)

作     者：Iwona Deszcz Anna Lis-Nawara Piotr Grelewski Szymon Dragan Julia Bar 

作者机构：Department of Immunopathology and Molecular BiologyWroclaw Medical UniversityBujwida 4450-345 WroclawPoland Department and Clinic of Orthopedic and Traumatologic SurgeryWroclaw Medical UniversityBujwida 4450-345 WroclawPoland 

出 版 物：《Regenerative Biomaterials》 (再生生物材料（英文版）)

年 卷 期：2020年第7卷第6期

页      面：543-552页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 0805[工学-材料科学与工程（可授工学、理学学位）] 10[医学] 

基　　金：supported by the Wroclaw Medical University grant no.STM.B132.17.012 

主　　题：articular cartilage regeneration chondrogenesis direct co-culture Hyaff-11 

摘      要：This study presents direct 2D and 3D co-culture model of mesenchymal stem cells(MSCs)line with chondrocytes isolated from patients with osteoarthritis(unaffected area).MSCs differentiation into chondrocytes after 14,17 days was checked by estimation of collagen Ⅰ,Ⅱ,Ⅹ,aggrecan expression using ***,localization of cells on Hyaff-11 was performed using enzymatic technique and THUNDER Imaging *** showed,that MSCs/chondrocytes 3D co-culture induced suitable conditions for chondrocytes grow and MSCs differentiation than 2D *** that differentiated cells on Hyaff-11 expressed collagen Ⅹ,they showed high collagen Ⅱ(80%)and aggrecan(60%)expression with simultaneous decrease of collagen Ⅰ expression(10%).The high concentration of differentiated cells on Hyaff-11,indicate that this structure has an impact on cells cooperation and *** conclusion,we suggest that high expression of collagen Ⅱ and aggrecan in 3D co-culture model,indicate that cooperation between different subpopulations may have synergistic impact on MSCs chondrogenic *** the high concentration and localization of cells growing in deeper layers of membrane in 3D co-culture,indicate that induced microenvironmental enhance cell migration within ***,we suggest that co-culture model might be useful for construction a bioactive structure for cartilage tissue regeneration.