Effect of quercetin on chondrocyte phenotype and extracellular matrix expression

作     者：GUI Zhi-Peng HU Yue ZHOU Yu-Ning LIN Kai-Li XU Yuan-Jin GUI Zhi-Peng;HU Yue;ZHOU Yu-Ning;LIN Kai-Li;XU Yuan-Jin

作者机构：Department of Oral&Cranio-maxillofacial SurgeryShanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai 200000China National Clinical Research Center for Oral DiseasesShanghai Key Laboratory of Stomatology&Shanghai Research Institute of StomatologyShanghai 200000China Department of Oral SurgeryShanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai 200000China 

出 版 物：《Chinese Journal of Natural Medicines》 (中国天然药物（英文版）)

年 卷 期：2020年第18卷第12期

页      面：922-933页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(81771038,82071082) Sciece and Technology Commission of Shanghai Municipality(18441902900,19142202200) 

主　　题：Chondrocyte Phenotype Quercetin Silk protein Cartilage Regeneration 

摘      要：Due to the poor repair ability of cartilage tissue,regenerative medicine still faces great challenges in the repair of large articular cartilage *** is widely applied as a traditional Chinese medicine in tissue regeneration including liver,bone and skin ***,the evidence for its effects and internal mechanisms for cartilage regeneration are *** the present study,the effects of quercetin on chondrocyte function were systematically evaluated by CCK8 assay,PCR assay,cartilaginous matrix staining assays,immunofluorescence assay,and western *** results showed that quercetin significantly up-regulated the expression of chondrogenesis genes and stimulated the secretion of GAG(glycosaminoglycan)through activating the ERK,P38 and AKT signalling pathways in a dose-dependent ***,in vivo experiments revealed that quercetin-loaded silk protein scaffolds dramatically stimulated the formation of new cartilage-like tissue with higher histological scores in rat femoral cartilage *** data suggest that quercetin can effectively stimulate chondrogenesis in vitro and in vivo,demonstrating the potential application of quercetin in the regeneration of cartilage defects.